ISOLATION AND CHARACTERISATION OF MICRO-ORGANISMS WITH INDUSTRIAL IMPORTANCE FROM SISAL BOLE ROTS

Abstract

Investigation of microorganisms naturally acclimatized to Agave hybrid H 11648 (sisal bolerot) was conducted, with the aim of isolating and characterizing Aspergillus niger strainsfor industrial use. Microorganism were identified morphologically and then confirmationmade by polymerase chain reaction (PCR). Results showed the existence of four majorgroups, listed in order of abundances as follows; Aspergilli (36.0 ±0.8) %, Penicillin(28.0 ±0.1) %, Yeast (15.0 ±1.6) %and Fusarium (10.0 ±0.12) %. The main groups ofAspergilli strains were A. nidulans, A. tamari and A. niger in ratios (3:2:2), respectively.Several endo-spore forming non-enteric gram (-) rods and coccid bacteria identified by API20 NE identification systemincluded,Brevundimonas diminuta sp, Shewanella putrefacienssp, Brevundimonas vesicularis sp and Pasteurella sp. Results showed that sisal bole rotstems hosts a high bio-diversity of microorganism species other than A. niger. Exploitationof the individual strains is recommended. This could eventually produce strains forprecursors of industrially and therapeutically metabolites.